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WebOct 1, 2024 · Based on the cellular compartment to be targeted to, the BirA gene was cloned with the HA-tag alone for expression in the cytosol, or with a CRT signal sequence at the N-terminus and the HA-tag and ER retention sequence, KDEL, at the C-terminus for localizing it to the ER [15].The compartmentalization of BirA allows biotinylation of BAP-tagged … WebAug 30, 2024 · Directions. Place 2/3 cup milk and water in a microwave-safe bowl and microwave for 30 seconds; the mixture should be lukewarm. Sprinkle yeast and sugar over the warmed milk mixture and let sit until frothy, about 3 minutes. Sift salt with flour into a … crab cakes recipe with canned crab
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