Binary ethylenimine virus inactivation
WebThe reduced inactivation rates for binary ethylenimine directly prepared in the virus suspension are due to the different cyclization rates of 2-bromoethylamine hydrobromide and 2-chloroethylamine hydrochloride and to the interference of bicarbonate in the cyclization reaction. WebOct 1, 2001 · The inactivation is expressed in reduction factors (RF): RF = log 10 initial virus titre virus titre after inactivation. 2.5. Protein modifications Test proteins (myoglobin, ovalbumin, BSA; 150 μM) were incubated in the presence of ethyleneimine (20 mM) at different pH values (6–8) for 24 h at room temperature.
Binary ethylenimine virus inactivation
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WebBinary ethyleneimine (BEI) is an aziridine compound, produced from bromoethylamine hydrobromide (BEA) commonly used for the inactivation of FMD virus during … WebAbstract. Experiments were carried out to inactivate F.M.D. viruses with the use of binary ethylene-imine. It was found that inactivation was optimal when the agent was used at …
WebKeywords: avian influenza virus subtype H5N1, binary ethylenimine, hemagglutination activity, virus inactivation 1 Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai ... WebAvian influenza virus (AIV) subtype H5N1 is known as a highly pathogenic agent for man and various kinds of animals, especially avian species. Herein, AIV H5N1 was inactivated by binary ethylenimine (BEI), an aziridine compound, produced from 2-bromoethylamine hydrobromide. BEI was used at concentrations of 0.001 and 0.01 M for AIV inactivation.
WebJun 27, 2011 · Measurement of PRRSV-specific antibody levels by IDEXX ELISA after vaccination according to the viral titer. For the experimental efficacy test according to the viral titer, PRRSV suspensions of titers of 10 4, 10 5, or 10 6 PFU/mL were inactivated with binary ethylenimine (BEI) and inoculated into pigs. From 21 to 56 days after the first … WebThe inactivation rates with binary ethylenimine prepared apart from the virus suspension in dilute sodium hydroxide with either 2-bromoethylamine hydrobromide or 2 …
WebAug 1, 1990 · The preparation and application of the aziridine compound binary ethylenimine (BEI) and the necessary conditions for and controls of the inactivation …
WebInactivation of Newcastle Disease Virus (NDV) by binary ethylenimine (BED is reported. The activity of an oil vaccine prepared with BEI-inactivated NDV was compared to a … should the driving age be increased to 18WebBinary ethylenimine (BEI) is a preparation of aziridine. It can be produced by heating bromoethylamine hydrobromide or 2-aminoethyl hydrogen sulfate in the presence of … should the drinking age be increasedWebSep 1, 2005 · The rabies vaccine is produced by inactivation of rabies virus propagated on BHK 21 cells. In the rabies inactivation process, BEI is added at a final concentration of 1.6. mM to the viral harvest at 37 °C, followed by a second dose of BEI at 24 h post-inactivation.. Inactivation was confirmed by the mice innocuity test and tissue culture … should the drinking age stay at 21WebSep 1, 2004 · Binary ethyleneimine (BEI) is an aziridine compound, produced from bromoethylamine hydrobromide (BEA) commonly used for the inactivation of FMD virus during vaccine manufacturing. The... should the driving age be increasedWebOct 1, 2001 · Virus inactivation by ethyleneimines was first introduced more than 30 years ago. Selective targeting of nucleic acids was reported for oligomeric ethyleneimines. In … should the drinking age changeWebJan 1, 2024 · BEI inactivation procedure. The inactivation was conducted using 3 BEI concentrations (0.5 mM, 1.0 mM, and 1.5 mM) and a 2-dose application with the second … should the drinking age lowered to 18WebMay 1, 2006 · In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from... should the elgin marbles be returned