Fk2 antibody

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August undefined, 2024

WebFeb 21, 2014 · The third method utilized the anti-ubiquitin antibody FK2. In the initial studies, the FK2 method was the most efficient method of isolating endogenously ubiquitinated protein complexes and recognizing monoubiquitinated and … WebUbiquitin Antibody (FK2) [Unconjugated] Summary. This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and …

Anybody use anti-ubiquitin antibody for western blotting. Suggest ...

http://www.utopbio.cn/archives/date/2024/04/14 WebImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. north america organic coffee market

Clone FK2 Antibody Products Biocompare

WebGeneral description. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated proteins. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated ... WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight. north america or north-america

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The dynamics of histone H2A ubiquitination in HeLa cells

WebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) … WebJan 7, 2024 · The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterized by one-dimensional Western blotting. The immunoglobulin … north america oregonWebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : how to repair foundation cracks diy

"WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be … " - Fk2 antibody

Fk2 antibody

MilliporeSigma anti-Ubiquitin Clone: FK2, 100μg, Unlabeled:Antibodies …

Web11 rows · Anti-Ubiquitinylated proteins Antibody, clone FK2. Background Information. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a … WebBlock: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody (SMC-550) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti …

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WebApr 14, 2024 · Ubiquitinated proteins, detected by the monoclonal antibody FK2 (ub-FK2), and RNF8-dependent ubiquitinated H2A (ub-H2A, detected by monoclonal antibody E6C5) are localized on the sex body area (Lu et al., 2010). K48- and K63-linked poly-ubiquitins are enriched in the XY body (Lu et al., 2010). The role of ubiquitination around the XY body ... WebApr 14, 2024 · Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) 单泛素/多泛素偶联物单抗（FK2）品牌：Enzo; 13HP020AN-东洋一次性针头式过滤器0.2um孔径; 月桂硫酸盐肉汤培养基-其它; Whatman 滤膜 6720-5001 盘式滤器 Polydisc TF, 0.1m 10/盒

WebUbiquitin modification of p62 was detected using an antibody recognizing mono- and poly-ubiquitin (FK2) (lower panels). The EGFP–TRIM proteins were detected using an anti-GFP antibody. * indicates mono-ubiquitylated p62 and a high-molecular-mass p62. WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the …

WebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... WebRibosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is …

WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS. 分析证书.

WebAnti-Ubiquitinylated proteins Antibody, clone FK1 04-262 Sigma-Aldrich Anti-Ubiquitinylated proteins Antibody, clone FK1 Download Zoom Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against … north america osteoporosis treatment marketWebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... how to repair folding mirrors peugeot 3008WebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking. north america or north americanWeb(BML-PW8810-0500) Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2), 500 µg - Enzo Life Sciences - CiteAb 35 citations – A mouse monoclonal … north america or north americaWeb11 rows · Dilute to working strength with phosphate buffered saline pH 7.2-7.4. This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use … how to repair formica laminateWebJan 7, 2024 · antibody product name : Polyubiquitinylated conjugates monoclonal antibody (FK1) catalog : BML-PW8805-0500 quantity : 500 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK1 reactivity : spiny dogfish, human, mouse, rat, fruit fly application : western blot, ELISA, immunohistochemistry, immunocytochemistry, … north america outline printableWeb(C) Ubiquitin IP with the FK2 antibody from the cells expressing the indicated proteins, followed by IB with FK2 and the pan-ubiquitin antibody #3933. (D) IP using the HA antibody from the cells co-expressing HA-ubiquitin with the indicated proteins, followed by IB with the HA and pan-ubiquitin antibodies. north america outdoor institute